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Opinii şi recomandări curente pentru utilizarea meşei în chirurgia ginecologică – review al literaturii
Introduction
Breast cancer is the most common form of cancer in women in many countries of the world(1). It is well known that breast cancer, even when it represents a small mass, can metastasize and, frequently, when the tumor becomes palpable, there are already metastases(2-4). Some authors argue that breast cancer should be considered a systemic disease from the time of diagnosis(4-6).
Although the possibility of detection of metastases is technically limited, their discovery is crucial for defining the stage of the disease and for establishing the optimal treatment scheme.
Statistically, the most common sites of breast cancer metastases are the bones, brain, lungs and liver, but metastases may also occur in other places, such as the genital organs(7-9).
Because, in addition to local expansion, the occurrence and development of metastases are ones of the main factors that determine the death of the patient, scientists have been trying for a long time to decipher the mechanisms of metastasis.
“Why metastases occur in certain organs?” and “What are the conditions that must be met for their occurrence?” are the main questions. In the last decades, scientists are gathering evidence that metastasis is not a random process; there is an organ tropism with the implication of tissular mediators and also metastatic cells acquire important genetic and epigenetic new characteristics(10-12).
The purpose of this case presentation is to highlight some of the characteristics of cells from metastases in the uterus and ovaries of breast cancer.
Case presentation
A 62-year-old patient was hospitalized in the Gynecology Department of the Bucur Maternity – “Sf. Ioan” Emergency Clinical Hospital, Bucharest, for further investigations after a control computed tomography (CT) scan detected abnormal changes in myometrium and the uterine cavity.
From the patient history, we found that, four years before, she was diagnosed with a breast tumor, about 3.5 cm in diameter, located in the left breast, which infiltrated the adjacent skin. The tumor was biopsied and at the histopathological exam it was established the diagnosis of poorly differentiated breast ductal carcinoma.
At that time, immunohistochemical (IHC) exam was also performed and there were identified: estrogen receptors (ER) positive in 15-20% of cells; progesterone receptors (PGR) positive in 35-40% of cells, and the nuclear protein Ki67 positive in 1-2% of cells. The epidermal growth factor receptors (EGFR) and human epidermal growth factor receptor 2 (HER2) were negative.
According to the initial investigations, the disease was classified in stage IIIB (T4 N2 M0). Polychemotherapy was administered for the next three months, but after this treatment, at a control CT, images suggestive of bone and lung metastases appeared. This implied the reclassification to a presumed stage IV of disease (T4a N2 M1).
Left mastectomy and axillary lymphadenectomy were performed. The histopathological examination of the tumor and of the removed lymph nodes confirmed the diagnosis of invasive breast carcinoma, ductal type, poorly differentiated, with overlying skin and nipple invasion, and metastases in axillary lymph nodes, some overcoming ganglion capsule. The postoperative staging was pT4b pN1b PMX G3. The immunohistochemical exam identified ER positive in 85-90% of cells, PGR positive in 65-70% of cells, and Ki67 positive in 2-4% of cells. HER2 was negative.
After the surgery, the patient followed six series of polychemotherapy with 270 mg of paclitaxel and 570 mg of carboplatin once every 21 days, osteoclast inhibitors treatment, using 6 mg of Bondronat® monthly for an eight-month period, and 50 mg of Bondronat® a day for the following two months; 1600 mg of Bofenos® a day for one year, and hormone therapy with an aromatase inhibitor – anastrozol 1 mg/day for three years. During the course of polychemotherapy, the patient received 500 µg of a-darbepoetinum three weeks for chemotherapy-induced anemia, and 6 MU/series of pegfilgrastim, prophylactic for leukopenia. After two years, despite the treatment, a CT scan showed that the suspicious bone foci seemed to be steadily increasing in number.
Taking into account the patient’s history, the abnormal changes in the myometrium and uterine cavity, detected by the new control tomography scan, the suspicion of uterine metastases arose. Thus, aiming a biopsy, a uterine cavity curettage was performed. The histopathological examination of collected tissue fragments identified abnormal cells arranged in stroma in small groups, nests, and rows, irregularly shaped, with quantitatively reduced eosinophilic cytoplasm, and small hyperchromic nuclei with polar localization.
Consequently, the patient underwent hysterectomy with bilateral anexectomy. The histopathological exam identified in the uterine wall and also in the ovaries metastases of breast carcinoma.
The immunohistochemical examination discovered: ovarian metastases positive for gross cystic disease fluid protein 15 (GCDFP15) in areas, and ER positive in 75%, PGR positive in 40%, HER2 negative, and Ki67 positive in 10-15% of the tumor cells; uterine metastases with E-cadherin negative, cytokeratin7 (CK7) positive, ER positive in 98%, PGR positive in the rare nuclei below 3%, HER2 negative, GCDFP15 positive in areas, Ki67 positive in approximately 30%, and Wilms tumor protein 1 (WT1) negative in tumor cells, with smooth muscle actin (SMA) positive in smooth muscle fibers but negative in tumor cells.
After surgery, the patient underwent six series of polychemotherapy and aromataze inhibitor (letrozolum) treatment.
The clinical course was favorable and, one year after imaging examinations, bone scintigraphy and CT scan revealed no pathological evolutionary processes.
In general, breast cancer metastases in the uterus are considered rare, but as early as 1980, a study based on autopsy results (by Hagemeister et al., quoted by J.M. Debois) warns that metastases on this site have a higher incidence than was previously known (between 2% and 15% of cases)(7).
On the other hand, by searching the primary tumors in cases with metastases in the uterus, it was discovered that 43% of them were from a breast tumor(8).
The most frequent histological form of breast cancer is the ductal carcinoma, but uterine metastases, in 80% of cases, are from its lobular form, which is rarer(13).
In relation to the structure of the uterus, metastases are most frequently located in the myometrium (64% of cases), in the endometrium in only 4% of cases and, in the remaining 32% of cases, simultaneously in both the myometrium and endometrium(14).
In the presented case, CT scan raised the suspicion of uterine metastases. The biopsy by curettage could not establish with certainty the nature of the uterine tumor, due to its location in the uterine wall, even though initially it was suspected the endometrium involvement. The location in the uterine wall also explains the lack of symptoms(15).
The uterine metastases were associated with ovarian ones. Considering the higher frequency of ovarian versus uterine metastases, some researchers argued that uterine metastases originate from the ovarian metastases by lymphatic or hematogenous dissemination(9). However, the co-seeding mechanism from the primary tumor cannot be excluded.
The phenotypic differences, with regard to the immunohistochemical characteristics, between the primary tumor and the metastases, have been reported in a very few studies in the last decades(16,17). This immunohistochemical characteristics are not very well established and the reasons behind the existence of the phenotypic changes remains in the field of hypotheses.
The peculiarity of this case is that it enabled the analysis of phenotypic changes of the metastatic cells. Such cases are rare, and we can register significant immunohistochemical changes and new characteristics of cells in metastasis from ductal breast carcinoma in the uterus and ovaries (Table 1).
Analyzing the presented data, the first thing we notice is that, although the biopsy of the breast tumor was useful for the histological diagnosis, the data resulting from the immunohistochemical examination of the ER and PGR were clearly different compared to those obtained by the examination of the primary tumor. This raises questions about the usefulness of the immunohistochemical examination performed on small fragments of biopsy, in terms of guiding treatment and assessing the prognosis.
Regarding the IHC examination of the excised tumors, if the expression of ER knew no significant differences, instead there were large differences in the expression of PGR and nuclear protein Ki67. The PGR expression declined from 65-70% of cells in the primary tumor to 40% in the ovarian metastases and to 3% of cells in uterine metastases. Ki67 expression was increased from 2-4% of cells in the primary tumor to 10-15% in the ovarian metastases and up to 30% of cells in uterine metastases. Phenotypically, the registered differences signify the appearance of new cell lines, compared to those of the primary tumor, favored in terms of grafting and growth in a specific host organ.
The fact that PGR expression and the values of nuclear protein Ki67 in ovarian metastases represent an intermediary between primary tumor and uterine metastases may be another argument in favor of the hypothesis which claims that uterine metastases originate from the ovarian metastases. The frequency of this type of metastasis reported on autopsies shows that they are indicative of a distinct stage of disease progression and have important prognostic significance.
Conclusions
The occurrence of metastasis in uterus and ovaries is related to the phenotypic changes of the cells emerging from primary or secondary tumors.
The significant differences in the expression of immunohistochemical markers show that new cell lines, emerging from the primary tumor or other metastatic sites, are favored in terms dissemination, grafting and growing in a specific host organ.
The immunohistochemical examination, performed on small fragments of biopsy, in terms of guiding treatment and assessing the prognosis, can lead to a misjudgment of the case, and hence the need for this examination to be done on a sufficiently representative specimen.
In this particular case presented by us, most probably the uterine metastases originated from the ovarian metastases.
Conflicts of interests. The authors declare there are no conflicts of interest relating to or arising from this article and its content.
Consent. The written informed consent was obtained from the patient for the publication of this case report and accompanying images. A copy of the written consent is available for review by the Editorial office/Chief Editor/Editorial Board members of this journal.
Ethical approval and research ethics. The “Sf. Ioan” Emergency Clinical Hospital Ethical Committee approved this case report retrospective study (Approval No. 398/17.03.2019). All procedures performed in this study were in accordance with the ethical standards of the national research committee and with the Helsinki declaration and its latter amendments.
Authors’ contributions. Authors O.G. Olaru and C.V. Poalelungi managed the literature searches, designed the case presentation, performed the statistical analysis and wrote the manuscript. Authors A.D. Stănescu and O.D. Bălălău provided the clinical data, and authors L. Pleş and C. M. Pena managed the analyses of the presentation. All authors read and approved the final manuscript.
Acknowledgments. We thank Ariana Mihai who contributed to the tehnical review of the article.
carcinom ductal de sânmetastaze ovarienemetastaze uterine